Light-activated RNA interference in human embryonic stem cells
نویسندگان
چکیده
منابع مشابه
Light-activated RNA interference in human embryonic stem cells.
We describe a near infrared (NIR) light-activated gene silencing method in undifferentiated human embryonic stem cell (hESC) using a plasmonic hollow gold nanoshell (HGN) as the siRNA carrier. Our modular biotin-streptavidin coupling strategy enables positively charged TAT-peptide to coat oligonucleotides-saturated nanoparticles as a stable colloid formation. TAT-peptide coated nanoparticles wi...
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Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...
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Manipulation of gene function in embryonic stem cells by either over expression or downregulation is critical for understanding their subsequent cell fate. We have developed a tetracycline-inducible short hairpin RNA interference (shRNAi) for human embryonic stem cells (hESCs) and demonstrated doxycycline dose-dependent knockdown of the transcription factor OCT4 and the cell surface antigen bet...
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Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
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ژورنال
عنوان ژورنال: Biomaterials
سال: 2015
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2015.06.006